|
KMID : 0606920100180040454
|
|
Biomolecules & Therapeutics
2010 Volume.18 No. 4 p.454 ~ p.462
|
|
|
Neuroprotective Effects of Carpinus tschonoskii MAX on 6-Hydroxydopamine-Induced Death of PC12 Cells
|
|
Kim Min-Kyoung
Kim Sang-Cheol
Kang Jung-Il
Boo Hye-Jin
Hyun Jin-Won
Koh Young-Sang
Park Deok-Bae
Yoo Eun-Sook
Kang Ji-Hoon
Kang Hee-Kyoung
|
|
|
Abstract
|
|
|
|
The present study investigated the neuroprotective effect of Carpinus tschonoskii MAX and its intracellular protective mechanism on 6-hydroxydopamine (6-OHDA)-induced oxidative damage in PC12 cells. We found that pretreatment of PC12 cells with C. tschonoskii extract significantly inhibited the cell death induced by 6-OHDA in a dose dependent manner. C. tschonoskii extract decreased 6-OHDA-induced apoptotic events such as chromatin condensation, DNA fragmentation, the decrease of Bcl-2/Bax ratio, caspase-3 activation and PARP cleavage. C. tschonoskii extract also reduced generation of 6-OHDA-induced reactive oxygen species and nitric oxide. Furthermore, C. tschonoskii extract up-regulated the myocyte enhancer factor 2 D (MEF2D), a critical transcription factor for neuronal survival, and Akt activity, whereas it inhibited the activity of ERK1/2 and JNK. The results suggest that C. tschonoskii extract decreases 6-OHDA-induced oxidative stress and could prevent PC12 cell apoptosis induced by 6-OHDA via the up-regulation of MEF2D and Akt activity, and thus may have application in developing therapeutic agents for Parkinson’s disease.
|
|
|
KEYWORD
|
|
|
Carpinus tschonoskii MAX, 6-OHDA, PC12 cells, Apoptosis, MEF2D, Akt
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|